Welcome to the Biotage Peptide Synthesis Blogs.

      What is solid phase peptide synthesis?

      Sep 25, 2019 9:47:21 PM / by Elizabeth Denton posted in Biotage, Developments, synthesis, peptide, peptide synthesis, solid phase peptide synthesis, cleavage, synthesis tips, orthogonal protecting groups, synthesis optimization, method development, FMOC, BOC

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      More and more groups are exploring the utility of peptides with an ever widening variety of applications. And although peptides are getting cheaper to purchase outright, many groups are continuing to bring peptide synthesis in house. As more groups join the peptide community, I frequently encounter questions about the basics of peptide synthesis.

      In today's post, I'd like to cover a little history of solid phase synthesis as well as highlight some differences between the chemistries.

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      Room temperature allyl ester and alloc deprotections - what is the lifetime of palladium?

      Jun 11, 2019 8:30:33 PM / by Elizabeth Denton posted in solid phase peptide synthesis, orthogonal protecting groups, selecting deprotection, synthesis optimization

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      In a previous post, I did some work evaluating the efficiency of alloc removal with tetrakis palladium using microwave assistance and atmospheric conditions, which worked beautifully.  Given the known sensitivity of palladium catalysts (see Derek Lowe's post for a humorous dialogue), I sought to further explore the sensitivity of palladium towards the alloc removal in the context of a peptide.

      In this post, I'll explore a variety of atmospheric, room temperature alloc deprotection conditions aimed at evaluating the catalytic lifetime of palladium tetrakis for effective alloc removal. 

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      Using microwave heating to expedite your allyl ester or alloc deprotection

      Jun 11, 2019 8:28:51 PM / by Elizabeth Denton posted in peptide, peptide synthesis, solid phase peptide synthesis, orthogonal protecting groups, synthesis optimization

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      Orthogonal amino acid protecting groups effectively expand the chemical tool kit available to peptide chemists allowing for synthesis of much more complex molecules.  Often times, orthogonal protecting groups are used in Fmoc-based chemistry to facilitate post-synthesis modifications of peptides, like the addition of small molecule fluorophores and more commonly now, peptide cyclization efforts.

      In a previous post, I discussed optimizing the removal of an ivDde protecting group.  In today’s post, I’ll explore the removal of an alloc protecting group from a lysine residue.

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