Would you ever consider an alternative to reversed- phase HPLC to purify your synthetic peptides? It seems like a silly question, right. And like many of you, I literally laughed at my Product Manager when he asked me this same question in my first days at Biotage.
Fast forward a few years and my answer to that question is now very different. For those of you that have followed this blog, you’ll know that I have switched to reversed-phase flash chromatography almost exclusively for my peptide purification. In today’s post, I’ll highlight some of the critical reasons that have influenced my change in mindset.