As a peptide chemist, I was trained to purify my peptides with reversed-phase HPLC, just as many a peptide chemist before me. Despite the hundreds of hours I’ve logged in front of an HPLC, injecting samples and collecting peak fractions, I can’t imagine using any other method to purify my freshly synthesized and cleaved peptides. In fact, you’d be hard pressed to convince me to try something else. But here I am, trying something new. Wish me luck!
In this post, I’ll describe my experiences using flash chromatography to purify a new peptide sample.