Welcome to the Biotage Peptide Synthesis Blogs.

      How does media pore size impact peptide resolution?

      Sep 27, 2019 3:19:01 PM / by Elizabeth Denton posted in Chromatography Fundamentals, Peptides, Reversed-phase, Green, Sfär, Media and Resin, Cost, V-10, HPLC

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      Purification by reversed-phase chromatography relies primarily on a hydrophobic interaction of the molecule with the alkyl chains bonded to the stationary phase for column retention and elution through a partitioning mechanism.  While this is certainly true for purification of peptides, surface area accessibility and media particle size also play critical roles in the resolving power of a particular stationary phase.  The particle size influences the loading capacity, however pore size greatly influences molecular accessibility and therefore resolving power.

      In today’s post, I will demonstrate how pore size can impact your peptide purification using flash column chromatography.

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      Does loading method influence my peptide recovery after purification?

      Jul 17, 2019 6:25:29 PM / by Elizabeth Denton posted in Chromatography Fundamentals, Peptides, Reversed-phase, Solvents, Workflow, Sfär, Loading Techniques, V-10, Isolera

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      In peptide purification, sample loading onto the column is rarely considered.  Most, if not all, HPLC instruments come equipped with a sample injection loop which demands a liquid injection of the sample for purification.  If you decide to use flash chromatography to purify your peptides though, liquid injection is no longer the exclusive method for sample introduction to the column.  Alternatively, dry loading crude material is a strategy often used in small molecule purification, particularly when sample solubility concerns arise.

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      Post synthesis workup: What steps are necessary and what aren't?

      Jun 19, 2019 5:35:32 PM / by Elizabeth Denton posted in Peptides, Troubleshooting and Optimization, Synthesis, Workflow, V-10

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      You’ve just finished a peptide synthesis and now it’s time to cleave the peptide from the resin. You’ve selected a specific cleavage cocktail, performed the reaction and now what? The vast majority of peptide chemists will precipitate their peptide using an ether solution, lyophilize, and move on to purification. But is that the only option?

      In today’s post I’ll highlight an alternative strategy that saves both processing time, potentially dangerous reagents, all without compromising the integrity of the recently synthesized peptide.

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      Removing Peptide Cleavage Cocktail with Biotage® V-10 Touch

      Mar 28, 2019 3:15:58 PM / by Raffaella Bombarda posted in Peptides, V-10

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      From peptide synthesis to cleavage to dried peptide using the Biotage suite of tools. Ph. D. Elizabeth Denton takes the journey in 3 minutes.

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      Why Evaporating DMSO is Not a Problem Anymore in Peptide Synthesis

      Mar 19, 2019 3:07:27 PM / by Sarah Moran posted in Peptides, Solvents, V-10

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      Our seasoned peptide chemist Dr. Elizabeth Denton has tried it all when it comes to peptides. In this short video she shows that using DMSO (dimethyl sulfoxide) in a peptide synthesis workflow is perfectly fine together with the Biotage® V-10 Touch evaporator. Watch her evaporate it in just over eight minutes.

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      How St. John's University Accelerated their Cancer Research with the Biotage Peptide Workflow Solution

      Jan 11, 2019 4:14:31 PM / by Sarah Moran posted in Peptides, Synthesis, Workflow, Sfär, Customer case, Alstra, V-10, Isolera

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      "How can I take a two hour purification using old-school chromatography, and shorten it?" was the question Dr. Aaron Muth at St. John's University in New York asked himself. "By using the Biotage Isolera, I could take it down to 10 or 15 minutes."

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