Welcome to the Biotage Peptide Synthesis Blogs.

      Peptide Workflow in action at Red Glead Discovery

      May 28, 2020 3:33:10 PM / by Amit Mehrotra posted in Peptides, Workflow, Alstra

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      Red Glead Discovery (RGD) is a pre-clinical drug discovery CRO offering a broad range of services to Life Science clients. With a focus on small molecules and peptides, their drug discovery platform ranges from medicinal chemistry and synthesis to ADME and biology. In addition to the CRO business, they also perform research collaborations with various biotech and academic partners.

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      Peptide Synthesis - What do you need to get started?

      Nov 22, 2019 5:43:59 PM / by Elizabeth Denton posted in Peptides, Workflow, Pillar Page

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      More and more we are seeing groups that would historically undertake only traditional organic chemistry or possibly biochemistry/biology, incorporate peptides into their research programs.  While this is good for expanding the application scope and diversity in the peptide space, bringing synthesis in house can be a daunting undertaking. 

      In today's post, I'll talk about what it takes to get a peptide synthesis operation up and running, with a few considerations along the way.

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      What is solid phase peptide synthesis?

      Sep 25, 2019 9:47:21 PM / by Elizabeth Denton posted in Peptides, Synthesis, Workflow, Media and Resin, Pillar Page

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      More and more groups are exploring the utility of peptides with an ever widening variety of applications. And although peptides are getting cheaper to purchase outright, many groups are continuing to bring peptide synthesis in house. As more groups join the peptide community, I frequently encounter questions about the basics of peptide synthesis.

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      Does loading method influence my peptide recovery after purification?

      Jul 17, 2019 6:25:29 PM / by Elizabeth Denton posted in Chromatography Fundamentals, Peptides, Reversed-phase, Solvents, Workflow, Sfär, Loading Techniques, V-10, Isolera

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      In peptide purification, sample loading onto the column is rarely considered.  Most, if not all, HPLC instruments come equipped with a sample injection loop which demands a liquid injection of the sample for purification.  If you decide to use flash chromatography to purify your peptides though, liquid injection is no longer the exclusive method for sample introduction to the column.  Alternatively, dry loading crude material is a strategy often used in small molecule purification, particularly when sample solubility concerns arise.

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      Post synthesis workup: What steps are necessary and what aren't?

      Jun 19, 2019 5:35:32 PM / by Elizabeth Denton posted in Peptides, Troubleshooting and Optimization, Synthesis, Workflow, V-10

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      You’ve just finished a peptide synthesis and now it’s time to cleave the peptide from the resin. You’ve selected a specific cleavage cocktail, performed the reaction and now what? The vast majority of peptide chemists will precipitate their peptide using an ether solution, lyophilize, and move on to purification. But is that the only option?

      In today’s post I’ll highlight an alternative strategy that saves both processing time, potentially dangerous reagents, all without compromising the integrity of the recently synthesized peptide.

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      Peptide Workflow by Biotage

      Mar 28, 2019 3:47:33 PM / by Elizabeth Denton posted in Peptides, Workflow

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      What is the main goal of a peptide chemist? Elizabeth Denton, Ph.D., explains how Biotage sees the peptide synthesis workflow and how we focus on shortening the process time for scientists.

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      How St. John's University Accelerated their Cancer Research with the Biotage Peptide Workflow Solution

      Jan 11, 2019 4:14:31 PM / by Sarah Moran posted in Peptides, Synthesis, Workflow, Sfär, Customer case, Alstra, V-10, Isolera

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      "How can I take a two hour purification using old-school chromatography, and shorten it?" was the question Dr. Aaron Muth at St. John's University in New York asked himself. "By using the Biotage Isolera, I could take it down to 10 or 15 minutes."

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