Ever experience the problem where you load your dissolved sample into a flash column via a syringe only to encounter either resistance or “blowback”? Blowback is a term describing the situation where your sample does not stay in the column but flows back out of the column’s cap after the loading syringe is removed.
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In today’s post I’d like to discuss how you can check your Biotage flash system to ensure it is running smoothly and still performing at the high level and capability that is expected of all Biotage products. I’ll go over how to set up and run the Sfär-TM1 parabens test, what to look for as indicators of the “health” of the system, and what you can do if anything looks amiss.
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Flash chromatography can be complex. Solvent choice, column size, stationary phase, loading technique, gradient method, flow rate, and detection parameters are all variables which factor into flash chromatography and your success with this purification technique. Of these variables, detection parameters, i.e. the type of detector, can really impact your flash chromatography results.
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