Welcome to the Biotage Flash Purification Blogs.

    Why is Solvent Evaluation by TLC Important for Good Flash Chromatography Results?

    November 22, 2022 at 3:27 PM / by Bob Bickler posted in TLC, selectivity, solvent

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    Organic chemistry can be messy with not only the desired compound created but also myriad by-products. As synthetic chemists, our goal is to make these desired compounds in sufficient yield and purity to either create another compound or submit for evaluation by other departments. To purify these reaction mixtures, flash chromatography is the primary technique.

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    How does a flash system’s internal volume affect gradients?

    November 8, 2022 at 9:21 PM / by Bob Bickler posted in gradient, volume

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    For many years I have been publishing a blog on flash chromatography, especially related to its theory and application. It dawned on me today that one area I have not yet addressed is the impact of the flash system’s internal plumbing volume on a programmed gradient, so, here it is.

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    How does an acidic pH affect reversed-phase chromatography separations?

    November 1, 2022 at 2:19 PM / by Bob Bickler posted in Reversed-phase, flash chromatography, separation

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    Chromatography is as much an art as it is a science. Between synthetic reaction products and natural products, the range of compounds requiring separation, purification, and isolation is broad and diverse creating challenges from time to time. Because of this diversity, not all chromatographic separations can be performed with a “neutral” solvent system – one without added pH modifiers or buffers.

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