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Bob Bickler

Which solvents are best for terpene flash chromatography?

July 6, 2022 at 2:59 PM / by Bob Bickler

Well, it’s spring as I write this, my favorite time of year, and the foliage is in various stages of bloom. I like the rebirth of nature and the floral aromas that emanate from the blooms. These botanical aromas are the result of the various terpenes and terpenoids in the plant which can be very different between species.

While I don’t typically post on specific flash chromatography applications, apart from hemp, I felt the purification of these terpene compounds might be of interest, especially since the choice of chromatography solvent can dramatically impact not just their separation but also their detection.

So, how does the solvent choice impact terpene detection? Well, it is all related to the terpenes’ chemical structures, which are based on linked isoprene units, and the UV absorptivity of the chromatography solvents.

While many terpenes are saturated (no double bonds), low molecular weight compounds, most have at least one double bond, which will absorb UV in the 198-220 nm range. But that is where the problem lies, especially in regards to their chromatography.

Normal-phase chromatography, utilizing hexane and ethyl acetate (EtOAc) as the elution solvents, is problematic because ethyl acetate also absorbs UV significantly in the 198-220 nm wavelength range making the terpenes a challenge to detect, Figure 1.EtOAc UV spectrum

Figure 1. EtOAc UV spectrum show UV absorption from <200 nm to ~250 nm.

There are, however, other solvents with greater UV transparency at those lower wavelengths which can be used in place of EtOAc – acetone and methyl t-butyl ether (MTBE). Both solvents have high UV transmissivity in the needed terpene detection wavelength range of 198-220 nm, Figure 2.

MTBE UV spectrumAcetone UV spectrum

Figure 2. UV spectra of MTBE (left) and acetone (right). MTBE is fully transparent to UV light across all wavelengths while acetone has high UV transmissivity at lower wavelengths.


As an added benefit, MTBE is UV transparent throughout the wavelength range for conjugated and aromatic compounds (220-400 nm) unlike acetone’s strong absorption between 220 and 300 nm. Though in a different solvent selectivity group (I) than EtOAc and acetone (both group VI), MTBE has a similar solvent strength (0.43) meaning that compounds will have a similar elution volume at the same solvent ratio.

Great. How does all this impact the separation and isolation of terpene compounds? Well, let’s see.

I tested the different solvents for the purification of two very different botanical extracts – Lavender Augustafolia and Ylang Ylang II, both from Aura Casia. Lavender consists primarily of mono terpenes while Ylang Ylang contains sequiterpenes and mono terpenes.



Using a Biotage 5-gram Sfär HC silica column on a Biotage® Selekt flash system with Spektra software, I compared the three solvents using a 0-50% gradient detecting with a wavelength range of 198-200 nm, Figure 3.Lavender composite

Figure 3. Lavender purification using different solvents. Top - hexane/acetone. Middle - hexane/EtOAc. Bottom - hexane/MTBE.

It is quite easy to see that detection sensitivity with hexane/EtOAc is greatly reduced as are the number of detectable compounds compared to hexane/MTBE. While hexane/acetone provides better sensitivity, a major lavender compound was not detected which is also true with the ethyl acetate gradient.

Ylang Ylang

We see some of the same issues with the Ylang Ylang extract where detection sensitivity is greatly diminished vs. acetone and MTBE, Figure 4.Ylang composite

Figure 4. Ylang Ylang purification comparison. Top - hexane/acetone. Middle - hexane/EtOAc. Bottom - hexane/MTBE.

With this comparison we also see acetone and ethyl acetate eluting some of the extract compounds earlier than MTBE. This is likely due their being in a different selectivity group (group VI) versus that of MTBE (group I). We know this because the broad red peak between 4 and 6 CV in the MTBE purification is the same compound as the red peak at 4 CV with the acetone chromatogram (and the blue peak at 4 CV of the EtOAc run). We can also see that there are more detected compounds when MTBE was used as the strong "B" solvent.

So, what have we learned?

  1. Chose purification solvents wisely and factor in their UV absorption
  2. While Biotage® Spektra software subtracts the solvent’s UV absorption, it can reduce the detection sensitivity of compounds also absorbing in the wavelength range of the solvent
  3. Solvent selectivity class can influence compound retention
  4. Experiment with different “B” or polar modifier solvents when developing methods

For more information on flash chromatography click below.

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CTA Successful Flash Chromatography (PPS490)

Topics: UV, selectivity

Bob Bickler

Written by Bob Bickler

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