Historically, solid phase peptide synthesis has been conducted at room temperature, demanding long reaction times and often double coupling to ensure a quality crude peptide product. More recently however, different strategies have been identified to heat the reactor vial, increasing the overall reaction rate and potentially the crude purity of your peptide.
In today’s post I will demonstrate that microwave heating can improve the crude purity of your desired peptide.
The relationship between temperature and reaction rate was identified by Arrhenius in 1889 and is defined by the following equation.
While many of us have used this equation ad nauseum during first year chemistry, the question immediately arises “what kind of heat?” Traditionally, hot plates and oil baths were the tools of the synthetic chemist for their reactions. Organic chemists began experimenting in the mid 1980s with microwave heating and found significantly decreased reaction times and increased reaction efficiencies when compared to conventional heating methods. This is attributed to the rapid, even, and controlled heating provided by a microwave source, Figure 1.
To highlight the differences that can be achieved when utilizing microwave heating during the coupling step, I synthesized an amphipathic peptide commonly referred to as 18A. For this comparison I kept the synthetic scale, coupling reagents, and number of equivalents constant while varying only reaction temperature and time.
I first synthesized 18A at room temperature with my Biotage® Initiator+ Alstra™, Figure 2. While it is common to double couple all amino acids during room temperature synthesis, I chose to single couple all amino acids in this synthesis for a more accurate comparison. The coupling reaction was allowed to proceed for 60 minutes, a relatively common length of time for room temperature syntheses.
I calculated the crude purity to be 59.5% by integrating the analytical HPLC UV trace. Despite the single couplings, this synthesis turned out to be more successful than I had initially anticipated.
I then synthesized the 18A using microwave heating for the coupling reactions with my Biotage Initiator+ Alstra, Figure 3. The coupling reactions proceeded for 5 minutes at 75ºC. It is important to note that for both peptides, I conducted the Fmoc-deprotection step at room temperature. There are several side reactions that can be exacerbated if the deprotection step is heated.
Visual inspection of the two chromatograms clearly shows that the peptide synthesized with microwave heating has a greater crude purity. I integrated the peaks and calculated the crude purity of this peptide to be about 67%, a less significant differential than I anticipated when I began this project. Importantly, a significant leading, yet poorly resolved, impurity present in the crude peptide mixture synthesized at room temperature is present to a lesser extent in the peptide mixture synthesized with microwave heating, simplifying the purification.
While there are certainly cases in which room temperature synthesis is appropriate, synthesis of peptide libraries for example, I generally recommend the use of microwave heating when possible. In addition to significantly reducing the required synthesis time (>36 hrs to <15 hrs), the use of microwave heating has also enabled synthesis of peptides with purities not previously accessible with room temperature approaches such as long peptides (>45 amino acids), phosphopeptides, and glycopeptides.
What heating strategies have you used to improve your crude peptide quality?